streptavidin alexa fluor 647 (Thermo Fisher)

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Thermo Fisher streptavidin alexa fluor 647
Streptavidin Alexa Fluor 647, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/alexa+fluor+647/Streptavidin%2C+Streptomyces+avidinii/pm42286068-427-13-16
Average 99 stars, based on 1 article reviews

streptavidin alexa fluor 647 - by Bioz Stars, 2026-09

99/100 stars

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Incubation:

Article Title: Extracellular vesicle-derived miRNA-182-5p educates macrophages towards an immunosuppressive phenotype in pancreatic cancer
Article Snippet: Afterwards, cells were blocked in 3% BSA for 15 mins and were incubated with appropriate primary antibodies (CD206, Proteintech, #60143-1 g, and PD-L1, Proteintech, #66248-1 g) overnight at 4 °C. .. The next day, the cells were gently washed with DPBS and incubated with Alexa Fluor 647 (Invitrogen., A32733) or Alexa Fluor 488 (Invitrogen., A32731TR) secondary antibodies, respectively, for 1 h at room temperature. .. Cells were then stained with DAPI, and images were captured using the Keyence BZ-X710 inverted fluorescent microscope (Keyence corporation).

Article Title: Graphene oxide increases the phototransduction efficiency of copolymeric nanoimplants and rescues visual functions in rat and pig models of Retinitis pigmentosa.
Article Snippet: To label Müller cells/ astrocytes, microglia and microglia activation, retinal sections were incubated with anti-GFAP (1:200, G3893, Sigma-Aldrich), anti-Iba-1 (cat# 019-19741; 1:300; Fujifilm Wako, Neuss, Germany) and anti- rat CD68 (1:250, MCA341R, BioRad Antibodies), respectively. .. To remove the excess primary antibodies, samples were rinsed 3 times in 0.1M PBS and then incubated for 1 h at room temperature with Alexa Fluor 488 (1:100 diluted in 0.05% Triton X-100 in 0.1MPBS), Alexa Fluor 647 (1:500, A21245, invitrogen) -conjugated secondary antibodies, and bisbenzimide nuclear labeling (1:300; Sigma-Aldrich). .. Samples were rinsed 3 times in0.1MPBS, dried, andmountedwith VectaMount (cat# H-5501-60; Vector Laboratories, Newark, CA) onto glass coverslips.

Article Title: Aurkb deficiency disrupts microglial development, homeostasis and hinders remyelination following cuprizone-induced demyelination
Article Snippet: The following primary antibodies were used: rabbit anti-Iba-1 antibody (1:500, Wako, Cat: 019–19741), mouse anti-Iba-1 antibody (1:400, Abcam, Cat: ab283319), rat anti-CD206 antibody (1:200, Bio-Rad, Cat: MCA2235), rat anti-BrdU antibody (1:400, Abcam, Cat: ab6326), mouse anti-phospho-Histone H3 (Ser10) antibody (1:200, Cell Signaling Technology, Cat: 9706S), mouse anti-APC (1:100, CC-1, Merck, Cat: OP80), rat anti-myelin basic protein (Mbp) monoclonal antibody (1:500, Abcam, Cat: ab7349), rabbit anti-degraded myelin basic protein (dMbp) antibody (1:2000, Millipore Sigma, Cat: AB5864), rabbit anti-Olig2 antibody (1:500, Proteintech, Cat: 13999-1-AP) and rat anti-CD68 antibody (1:500, Abcam, Cat: ab53444). .. After washing five times with PBS at room temperature, the sections were incubated with species-specific secondary antibodies conjugated to Alexa Fluor dyes: Alexa Fluor 488 (1:500, ThermoFisher Scientific, Cat: A-21206/A-11006), Alexa Fluor 555 (1:500, ThermoFisher Scientific, Cat: A-31572/A-21422) and Alexa Fluor 647 (1:500, ThermoFisher Scientific, Cat: A-32733), for 2 h at room temperature. .. TUNEL staining was performed according to the manufacturer’s protocol (Roche, Cat: 12156792910) following the removal of secondary antibodies.

Article Title: Extracellular vesicle-derived miRNA-182-5p educates macrophages towards an immunosuppressive phenotype in pancreatic cancer.
Article Snippet: Afterwards, cells were blocked in 3% BSA for 15 mins and were incubated with appropriate primary antibodies (CD206, Proteintech, #60143-1 g, and PD-L1, Proteintech, #66248-1 g) overnight at 4 °C. .. The next day, the cells were gently washed with DPBS and incubated with Alexa Fluor 647 (Invitrogen., A32733) or Alexa Fluor 488 (Invitrogen., A32731TR) secondary antibodies, respectively, for 1 h at room temperature. .. Cells were then stained with DAPI, and images were captured using the Keyence BZ-X710 inverted fluorescent microscope (Keyence corporation).

Next-Generation Sequencing:

Article Title: Dopamine and serotonin cotransmission filters striatonigral synaptic activity via 5-HT1B receptor activation.
Article Snippet: The primary antibodies were a rabbit anti- DARPP- 32 (Cell Signaling Technologies, 2306) diluted 1:500 and a chicken anti- GFP (Abcam, Ab13970) diluted 1:2000. .. The following day, after another three 10- min rinses in TBS, the sections were put in TBS containing goat- raised fluorescently conjugated Alexa Fluor 488, Alexa Fluor 568, and Alexa Fluor 647 (Life Technologies) secondary antibodies, and 2% NGS and 0.3% Triton- X100, for 45 min at room temperature. .. After three washes in TBS, the sections were mounted onto Superfrost microscope slides (Thermo Fisher Scientific) and Fluoromount- G anti- fading medium containing 4′,6- diamidino- 2- phenylindole (SouthernBiotech).

Labeling:

Saline:

Article Title: Mechanochemical waves in focal adhesions during cell migration
Article Snippet: Collapse of posts during peeling was rectified by sonication in 100% ethanol and critical point drying in CO 2 (Samdri-PVT-3D; Tousimis). .. For functionalization, flat PDMS stamps were coated with fibronectin [Thermo Fisher Scientific, D307; 50 μg/ml in phosphate-buffered saline (PBS)] and Alexa Fluor 647–labeled fibrinogen (Thermo Fisher Scientific, F35200 ; 20 μg/ml in PBS) for 1 hour, rinsed with water, nitrogen dried, and stamped onto oxidized mPADs (UVO-Model 342; Jelight). .. Surfaces were passivated with 0.2% Pluronic F-127 (Sigma-Aldrich, catalog no. P2443) for 30 min to prevent nonspecific binding.

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